Personally Dedicated to Making Your Embryo Transfer Program Successful

Embryo Preparation:

The embryo preparation process has been standardized by the International Embryo Transfer Society (IETS). The washing process, as well as the staging and quality grading processes all have scientifically proven guidelines that we use. By utilizing these standardized procedures the embryos we flush from a donor are qualified for potential export.

Embryo Washing Process

The washing process is vital to disease prevention. To date, there has been no disease transferred from donor to recipient via an embryo.

We use two different types of media, holding media and Trypsin, when preparing embryos. These medias have gone through a rigorous testing process and have been determined to create a favorable environment for the embryo.

Staging Embryos

The stage code signifies the relative developmental age of an embryo. Embryos that are seen at the time of flushing are typically morula (4), early blastocyst (5) or blastocyst (6).

Quality Grading Embryos

Embryos are given a quality grade base primarily on the amount of viable cell mass that is present in comparison to dead and degenerating cells. There are four quality grades. One, two, three and degenerate; where one is the best, three is an embryo that can be used in a fresh transfer but is not likely to survive the freezing (cryopreservation) process and a degenerate is an embryo that was fertilized but died and is now decomposing.

Laser Assisted Hatching

Laser assisted hatching (LAH) is the process by which a small hole is etched in the outside edge of the zona pellucida (zona or zp). This hole aids the embryo in breaking out of its zona. This process is most beneficial for in-vitro produced (IVP) embryos and Brahman breeds/Bos indicus. The advantages in embryos produced in-vivo in Bos taurus breeds is still being studied.

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